Light Microscopy

Olympus BX50 Light Microscope with QImaging Retiga 2000R digital camera.

The Olympus BX50 Light Microscope is an upright microscope with 4X, 10X, 20X, 40X (with collar correction for coverslip thickness), and optional 60X (oil) and 100X (oil) objectives. It is fitted with Mercury epi-fluorescence and Halogen direct illumination systems and binocular infinity optics eye pieces. The microscope is equipped with filter blocks to visualize 488 (excites blue, emits green, such as FITC), 568 (excites green, emits red, such as Rhodamine), with a multi filter cube which provides 488, 568 and UV light (used for DAPI). The blank setting is used for UV and bright field. Visit for further information.

Mouse aorta pic red polarized light     Mouse aorta oil red O transmitted light     Mouse aorta Sytox Green fluorescence

Papers published from the MIC:

Fonseca C, Taatjes DJ, Callas P, Ittleman F, Bovill EG. (2012) The effects of aging on the intimal region of the human saphenous vein: insights from multimodal microscopy and quantitative image analysis. Histochem Cell Biol. Sep;138(3):435-45.

Additional papers.

Features include:

  • Epi-fluorescence objectives (10X, 20X, 40X, 60X, 100X).
  • Phase Contrast.
  • Double and triple fluorescence staining possible.
  • Computer and software for image management and archiving.