Atomic Force Microscope
MFP-3D-BIOTM Atomic Force Microscope by Asylum Research is mounted on Olympus IX71 inverted microscope that resides on a Herzan AVI 350-S Active Vibration Isolation Table which dynamically dampens the most extreme vibrational disturbances; and the system is totally enclosed in an air tight state-of-the-art BCH-45 Large Acoustic Isolation Hood with air temperature control capabilities. The closed looped system allows investigation of dry or hydrated biological/materials surface features at very high resolution to include dynamic 3D visual analysis, force curves, optical (fluorescence and phase) image overlays precisely registered over AFM topographical information; plus advanced modes such as nanomanipulation(s), nanoindentation(s), Dual AC and more. The system software modules are written in Igor Pro, an integrated program by Wavemetrics which provides very advanced features for visualizing, analyzing, transforming and presenting experimental data.
Papers published from the MIC:
- Bezati, E., Wu, X-X., Quinn, A., Taatjes, D.J., and Rand, J.H. (2015) A new trick for an ancient drug: Quinine dissociates antiphospholipid immune complexes. Lupus 24, 32-41.
- Quinn AS, Rand JH, Wu XX, Taatjes DJ. (2013) Viewing dynamic Interactions of proteins and a model lipid membrane with atomic force microscopy. Methods Mol Biol. 931:259-93.
- Rand JH, Wu X-X, Quinn AS, Ashton AW, Chen PP, Hathcock JJ, Andree AAM, Taatjes DJ. (2010) Hydroxychloroquine protects the annexin A5 anticoagulant shield from disruption by antiphospholipid antibodies: evidence for a novel effect for an old antimalarial drug. Blood. 115, 2292-2299.
- Rand JH, Wu X-X, Quinn AS, Chen PP, Hathcock JJ, Taatjes DJ. (2008) Hydroxychloroquine directly reduces the binding of anthiphospholipid antibody-Beta2-glycoprotein I complexes to phospholipid bilayers. Blood. 112, 1687-1695.
- Montigny WJ, Quinn AS, Wu X-X, Bovill EG, Rand JH, Taatjes DJ. (2006) Atomic force microscopy in the study of macromolecular interactions in hemostasis and thrombosis: Utility for investigation of the antiphospholipid syndrome. Atomic Force Microscopy: Applications in Biology and Medicine (BP Jena and KH Horber, eds), John Wiley & Sons, Inc., Hoboken, NJ, pp. 267-286.
- Jeremic A, Quinn AS, Cho WJ, Taatjes DJ, Jena BP. (2006) Energy-dependent disassembly of self-assembled SNARE complex: Observation at nanometer resolution using atomic force microscopy. J. Am. Chem. Soc. 128, 26-27.
- Rand JH, Wu XX, Quinn AS, Chen PP, McCrae KR, Bovill EG, Taatjes DJ. (2003) Human monoclonal antiphospholipid antibodies disrupt the annexin A5 anticoagulant crystal shield on phospholipid bilayers: evidence from atomic force microscopy and functional assay. Am J Pathol. 163(3):1193-200.