Image Analysis Software

The MetaMorph software is on a dedicated computer using a 20 inch flat screen monitor with associated color printer. MetaMorph Offline software (Universal Imaging Corporation, a subsidiary of Molecular Devices) is a high end software package used extensively for image analysis, including colocalization, measurements of areas and distances and object counting and cell tracking with interactive user created regions of interest or automated analysis using an intensity threshold. All measurements are logged onto an Excel spreadsheet for subsequent analysis of data.

  • Colocalization
  • Object counting
  • Distance measurement
  • Area measurement
  • Cell tracking
  • 3D reconstruction

PerkinElmer Volocity 3D Rendering Software

Volocity (PerkinElmer, Waltham, MA) is a high-performance 3D imaging software package providing visualization, exploration and analysis capabilities of multi-channel 3D volumes over time. The software can be used for 2D, 3D, and 4D imaging, and will accept image sequences from both confocal and wide-field microscopes. The Volocity Visualization module provides an extensive array of visualization and publication features. The Volocity 3D view enables the user to interactively explore a 3D rendered object. The viewing point can be placed either inside or outside of the 3D object, and changes of structures over time monitored. Animated movie volumes can be created with the Movie Sequencer module. The Volocity Classification module will identify, measure and track biological structures in 2D, 3D, and 4D. Co-localization analysis can also be performed for two fluorophores in 3D.

MBF StereoInvestigator Software

StereoInvestigator (MBF Bioscience, Williston, VT) image analysis software is designed to count cells, quantify lengths, area, and volumes in biological samples while employing stereological principles. With 13 stereological probes, it will efficiently allow you to obtain precise and unbiased estimates of cell number (Optical Fractionator probe), volume fractions (Cavalieri Estimator probe), or spatial distribution (Nearest Neighbor probe) of tissue components. Results can be exported directly into Microsoft Excel.

MetaMorph: original and thresholded image

Volocity- biofilm reconstruction 

Mitochondria with Cavalieri markers