Transmission Electron Microscope
The JEOL 1400 Transmission Electron Microscope is a high-contrast, high-resolution instrument utilizing a LaB6 filament, and capable of operating at accelerating voltages up to 120 kV. It incorporates a newly designed electron optics system which is optimized for integrated use with a digital ccd camera imaging device. The instrument is controlled via the “TEM Center” graphical user interface operating environment, with redundant manual operation afforded by two conveniently located control panels. The image orientation system allows image rotation at multiple angles, and specimen stage movement is achieved with a precision trackball device. Software modules (JEOL Electron Microscope Navigation Interactive Engine: “JENIE”) provide step-by-step video tutorials covering instrument operation and alignment, especially useful for novice operators. Image acquisition provided by an AMT XR611 high resolution 11 megapixel mid-mount ccd camera. This camera is positioned near the normal film plane of the TEM affording a wide field of view for image capture. Moreover, the mid-mount configuration displays virtually no TEM distortion.
Papers published from the MIC:
- Naik, A. R., Kulkarni, S. P., Lewis, K. T., Taatjes, D. J., and Jena, B. P. (2016) Functional reconstitution of the insulin-secreting porosome complex in live cells. Endocrinology 157, 54-60.
- Yang, R., Lirussi, D., Thornton, T.M., Jelley-Gibbs, D.M., Gummadidala, P., Diehl, S.A., Case, L.K., Silverstrim, B., Madesh, M., Taatjes, D.J., O’Shea, J.J., Teuscher, C., Haynes, L., and Rincon, M. (2015) Mitochondrial Ca2+ and membrane potential, an alternative pathway for Interleukin 6 to regulate CD4 cell effector function. eLife 2015;4;e06376.
- Fang, J., Liu, M., Zhang, X., Sakamoto, T., Taatjes, D.J., Jena, B.P., Sun, F., Woods, J., Bryson, T., Kowluru, A., Zhang, K., and Chen, X. (2015) COPII dependent ER export: a critical component of insulin biogenesis and beta cell ER homeostasis. Mol. Endocrinol. 29, 1156-1169.
- Pishdadian K, Fimlaid KF, Shen A. (2015) SpoIIID-mediated regulation of sK function during Clostridium diffiile sporulation. Mol Microbiol. Jan;95(2):189-208.
- Fimlaid KA, Jensen O, Francis MB, Sorg JA, Shen A. (2015) Identification of a novel lipoprotein regulator of Clostridium difficile spore germination. PLoS Pathogens. Oct 23;11(10):e1005239.
- Fimlaid KA, Jensen O, Donnelly ML, Siegrist MS, Shen A. (2015) Regulation of Clostridium difficile spore formation by the SpoIIQ and SpoIIIA proteins. PLoS Genetics. Oct 14;11(10):e1005562.
- Wang, Y., Bishop, N.M., Taatjes, D.J., Narisawa, S., Millan, J.L., and Palmer, B.M. (2014) Sex-dependent, zinc-induced de-phosphorylation of phospholamban by tissue non-specific alkaline phosphatase in the cardiac sarcomere. Am. J. Physiol. Heart Circ. Physiol. 307, H933-H938.
- Rajagopal, A., Kulkarni, S., Lewis, K.T., Maarouf, A., Kelly, C.V., Taatjes, D.J., and Jena, B.P. (2014) Proteome of the insulin-secreting Min6 porosome complex: Involvement of Hsp90 in its assembly and function. J. Proteom. 114, 83-92.
- Hou X, Lewis KT, Wu Q, Wang S, Chen X, Flack A, Mao G, Taatjes DJ, Sun F, Jena BP. (2014) Proteome of the porosome complex in human airway epithelia: interaction with the cystic fibrosis transmembrane conductance regulator (CFTR). J Proteomics. Jan 16;96:82-91.
- Kovari LC, Brunzelle JS, Lewis KT, Cho WJ, Lee JS, Taatjes DJ, Jena BP. (2014) X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release. Micron. Jan;56:37-43.
- Hatle KM, Gummadidala P, Navasa N, Bernardo E, Dodge J, Silverstrim B, Fortner K, Burg E, Suratt BT, Hammer J, Radermacher M, Taatjes DJ, Thornton T, Anguita J, Rincon M. (2013) MCJ/DnaJC15, an endogenous mitochondrial repressor of the respiratory chain that controls metabolic alterations. Mol Cell Biol. Jun;33(11):2302-14.
- Cunniff B, Benson K, Stumpff J, Newick K, Held P, Taatjes D, Joseph J, Kalyanaraman B, Heintz NH. (2013) Mitochondrial-targeted nitroxides disrupt mitochondrial architecture and inhibit expression of peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells. J Cell Physiol. Apr;228(4):835-45.
- Lee JS, Hou X, Bishop N, Wang S, Flack A, Cho WJ, Chen X, Mao G, Taatjes DJ, Sun F, Zhang K, Jena BP. (2013) Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis. Micron. Apr;47:50-8.
- Fimlaid KF, Bond JP, Schutz KC, Putnam EP, Lawley TD, Shen A. (2013) Global analysis of the sporulation pathway of Clostridium difficile. PLoS Genetics. 9(8):e1003660.
- Putnam EE, Nock A, Lawley TD, Shen A. (2013) SpoIVA and SipL are Clostridium difficile spore morphogenetic proteins. J. Bacteriol. Mar;195:1214-1225.
- Wang S, Lee JS, Bishop N, Jeremic A, Cho WJ, Chen X, Mao G, Taatjes DJ, Jena BP. (2012) 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex. Histochem Cell Biol. Jun;137(6):703-18.
- Hillegass JM, Blumen SR, Cheng K, MacPherson MB, Alexeeva V, Lathrop SA, Beuschel SL, Steinbacher JL, Butnor KJ, Ramos-Nino ME, Shukla A, James TA, Weiss DJ, Taatjes DJ, Pass HI, Carbone M, Landry CC, Mossman BT. (2011) Increased efficacy of doxorubicin delivered in multifunctional microparticles for mesothlioma therapy. Int. J. Cancer. Jul;129(1):233-44.
Additional papers.