Transmission Electron Microscope

Transmission Electron MicroscopeThe JEOL 1400 Transmission Electron Microscope is a high-contrast, high-resolution instrument utilizing a LaB6 filament, and capable of operating at accelerating voltages up to 120 kV. It incorporates a newly designed electron optics system which is optimized for integrated use with a digital ccd camera imaging device. The instrument is controlled via the “TEM Center” graphical user interface operating environment, with redundant manual operation afforded by two conveniently located control panels. The image orientation system allows image rotation at multiple angles, and specimen stage movement is achieved with a precision trackball device. Software modules (JEOL Electron Microscope Navigation Interactive Engine: “JENIE”) provide step-by-step video tutorials covering instrument operation and alignment, especially useful for novice operators. Image acquisition provided by an AMT XR611 high resolution 11 megapixel mid-mount ccd camera. This camera is positioned near the normal film plane of the TEM affording a wide field of view for image capture. Moreover, the mid-mount configuration displays virtually no TEM distortion.

 

TEM image of kidney tubule    TEM image of muscle

 

 


Recent Papers published from the MIC:

  • Buskiewicz, I. A., et al. (2016). "Reactive oxygen species induce virus-independent MAVS oligomerization in systemic lupus erythematosus." Sci Signal 9(456): ra115.
  • Clemments, A. M., et al. (2015). "Protein Adsorption From Biofluids on Silica Nanoparticles: Corona Analysis as a Function of Particle Diameter and Porosity." ACS Appl Mater Interfaces 7(39): 21682-21689.
  • Cruz, F. F., et al. (2015). "Systemic Administration of Human Bone Marrow-Derived Mesenchymal Stromal Cell Extracellular Vesicles Ameliorates Aspergillus Hyphal Extract-Induced Allergic Airway Inflammation in Immunocompetent Mice." Stem Cells Transl Med 4(11): 1302-1316.
  • Guigni, B. A., et al. (2018). "Skeletal muscle atrophy and dysfunction in breast cancer patients: role for chemotherapy-derived oxidant stress." Am J Physiol Cell Physiol 315(5): C744-C756.
  • MacPherson, M., et al. (2017). "Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro." Histochem Cell Biol 147(5): 595-604.
  • Miller, M. S., et al. (2017). "Moderate-intensity resistance exercise alters skeletal muscle molecular and cellular structure and function in inactive older adults with knee osteoarthritis." J Appl Physiol (1985) 122(4): 775-787.
  • Munson, P., et al. (2018). "Exosomes from asbestos-exposed cells modulate gene expression in mesothelial cells." FASEB J 32(8): 4328-4342.
  • Munson, P., et al. (2018). "Mouse serum exosomal proteomic signature in response to asbestos exposure." J Cell Biochem 119(7): 6266-6273.
  • Munson, P. B., et al. (2019). "Exosomal miR-16-5p as a target for malignant mesothelioma." Sci Rep 9(1): 11688.
  • Ondzighi-Assoume, C. A., et al. (2016). "Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores." Plant Cell 28(3): 729-745.
  • Pappas, A. C., et al. (2015). "Astrocyte Ca2+ Signaling Drives Inversion of Neurovascular Coupling after Subarachnoid Hemorrhage." J Neurosci 35(39): 13375-13384.
  • Pearson, L., et al. (2018). "A Composite Renal Tumor with Dual Differentiation, Chromophobe and Collecting Duct Carcinoma." Case Rep Pathol 2018: 2410920.
  • Platz, J., et al. (2016). "Comparative Decellularization and Recellularization of Wild-Type and Alpha 1,3 Galactosyltransferase Knockout Pig Lungs: A Model for Ex Vivo Xenogeneic Lung Bioengineering and Transplantation." Tissue Eng Part C Methods 22(8): 725-739.
  • Ribis, J. W., et al. (2018). "Differential requirements for conserved peptidoglycan remodeling enzymes during Clostridioides difficile spore formation." Mol Microbiol 110(3): 370-389.
  • Ribis, J. W., et al. (2017). "The Conserved Spore Coat Protein SpoVM Is Largely Dispensable in Clostridium difficile Spore Formation." mSphere 2(5).
  • Thwe, P. M., et al. (2017). "Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses." Cell Metab 26(3): 558-567 e555.
  • Uhl, F. E., et al. (2017). "Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis." Methods Mol Biol 1627: 253-283.
  • Wrenn, S. M., et al. (2018). "Avian lungs: A novel scaffold for lung bioengineering." PLoS One 13(6): e0198956.
  • Yang, R., et al. (2015). "Mitochondrial Ca(2)(+) and membrane potential, an alternative pathway for Interleukin 6 to regulate CD4 cell effector function." Elife 4.

Additional publications located here.