Flow Cytometry and Cell Sorting (FCCS) Facility

The Harry Hood Bassett Flow Cytometry and Cell Sorting (FCCS) Facility at UVM is a College of Medicine Core Facility designed as a multi-user resource for the high speed analysis and sorting of cells. The FCCS Facility has BD LSRII and MACSQuant VYB analytical cytometers, and a BD FACS AriaIII high-speed cell sorter. This equipment was purchased through National Institute of Health and UVM-College of Medicine Shared Instrumentation Grants. Investigators who bring samples to the FCCS Facility for sorting or analysis pay a fee, which is used to offset operating expenses. The FCCS Facility provides professional consultation and assistance with equipment use, experimental design, and interpretation of flow data.

VYBfig_200x135Instrumentation

The FCCS Facility has BD LSRII and MACSQuant VYB analytical cytometers, and a BD FACS AriaIII high-speed cell sorter.

rates1Rates and Appointments

All fees are based on the amount of time scheduled. Analyzers have a minimum charge of 15 minutes, and cell sorting has a minimum charge of 30 minutes. 

tubesblack200x135Flow Cytometry Protocols

In this section you will find the most common Flow Cytometry Protocols that investigators at the UVM-FCCS Facility use.