Markus Thali, Ph.D.

Professor

Training & Education

Dr. Thali received his Ph.D. in Molecular Biology from the University of Zürich, Switzerland in 1990. He did postdoctoral research with Joseph Sodroski at the Dana-Farber Cancer Institute/Harvard Medical School and he was an Assistant Professor at the University of Lausanne before he joined the Department of Microbiology and Molecular Genetics late in 1999. 

Research Interests

Overall, my group is interested in understanding how information (genetic, biochemical) can be transmitted from cell to cell and how such information flow determines the fate of cells, tissues, and ultimately organisms. Recently, we have become particularly interested in the phenomenon that mere merging of cells (i.e. cell-cell fusion) can result in altered cell states.

The main object of our studies is the retrovirus HIV-1, though we also support investigations by colleagues who study other viruses or extracellular vesicles that serve as information carriers. Importantly, in all our projects we collaborate more or less extensively with other scientists, both on campus and at other institutions, thus forming transient alliances to tackle emerging questions.

While we use various virological and cell biological techniques for our investigations, particular emphasis is being placed on applying quantitative imaging methods. These include restoration fluorescence microscopy and super resolution microscopy for analyses of subcellular events and light sheet microscopy for analyses at the cell population level (with single cell resolution). Using such methods, we recently found that HIV-induced small T lymphocyte syncytia are a subpopulation of infected cells that have distinct surface protein profiles as well as migratory properties that distinguish them from infected mononucleated T cells. A major thrust of our current work thus aims at understanding what role this subpopulation of HIV-1-infected cells plays in virus spread. 

Featured Publications

Whitaker EE, Matheson NJ, Perlee S, Munson PB, Symeonides M, Thali M.  EWI-2 inhibits cell-cell fusion at the HIV-1 virological presynapse.  Viruses. 2019, 11, 1082.

Ikeda T, Symeonides M, Albin JS, Li M, Thali M, Harris RS. 2018. HIV-1 adaptation studies reveal a novel Env-mediated homeostasis mechanism for evading lethal hypermutation by APOBEC3G. PLoS Pathog 14:e1007010.

Symeonides M, Murooka TT, Bellfy LN, Roy NH, Mempel TR, Thali M. 2015. HIV-1-induced small T cell syncytia can transfer virus particles to target cells through transient contacts. Viruses 7:6590-6603.

Roy NH, Chan J, Lambele M, Thali M. 2013. Clustering and mobility of HIV-1 Env at viral assembly sites predict its propensity to induce cell-cell fusion. J Virol 87:7516-7525.

All Thali publications

Markus Thali, PhD

Contact Information

Office: 318B Stafford

Phone: 802-656-1056

Email

 

Lab Team

Post-doctoral Associate

Menelaos (Mel) Symeonides

Graduate Student

Emily Whitaker

Undergraduate Students

Elise Courtney
Ethan LaFrance
Brickley Littell
Matt Kinahan (in silico work only)