DNA Analysis Facility

The DNA Analysis Facility at the University of Vermont Cancer Center provides a wide array of efficient, user friendly, and affordable DNA analysis services to members of the University of Vermont Cancer Center, the University of Vermont research community, and outside institutions.

The facility provides a variety of important benefits free of charge to our users including experimental design consultation, data analysis and trouble shooting. Our annual Open House is a popular day long event highlighting our services and new technologies. The facility hosts a monthly Cancer Research and Technology Seminar series, presents software and instrumentation tutorials with the goal to educate and support our users, and showcase new services. A computer workstation area is adjacent to the main lab and has freeware and purchased programs available for user driven data analysis with full support from nearby facility staff.

Services

  • Automated DNA Sequencing
  • Cycle sequence reactions
  • Sequence runs
  • Fragment Analysis.
  • Genotyping, haplotyping, microsatellite markers
  • T-RFLP, AFLP
  • Human and Mouse Cell Line Authentication
  • Includes DNA extraction and microsatellite analysis 
  • Real time qPCR
  • Quantitative PCR (absolute or relative)
  • Allelic Discrimination Assays
  • SNP Detection (low or high-throughput)
  • RNA and DNA extractions
  • Nucleic Acid and Protein Quantification
  • Molecular Imaging
  • Consultation and Troubleshooting
  • Computer workstation with DNA analysis software

Instrumentation

  • ABI Prism 3100-Avant Genetic Analyzer (4 capillary)
  • ABI Prism 3130xl Genetic Analyzer (16 capillary)
  • ABI Prism 7900HT Sequence Detection System (Real-time qPCR)
  • AB 7500 Fast Real-time PCR System
  • BioRad Personal Molecular Imager FX
  • BioRad PharosFX Molecular Imager FX (with external Laser)
  • BioRad Versa Doc 4000 MP
  • BioTek Synergy H4 Hybrid Reader
  • DNA Engine 96-well gradient Thermal Cycler (2)
  • NanoDrop (2)

Computer Annex

The software accessible to users includes: GeneMapper, Sequencher, Chromas, Edit View, BioEdit, Quantity One, Primer Express, Primer Select, and Dissociation Curve software.