Research in Dr. Lounsbury's laboratory is focused on understanding mechanisms whereby signals generated at the plasma membrane are communicated to the nucleus to mediate vascular remodeling and tumor angiogenesis. Signaling pathways of current interest include protein kinase cascades, hypoxia signaling and mechanisms of calcium-regulated gene transcription in arterial smooth muscle cells and ovarian tumor cells. The laboratory uses a multidisciplined approach to study these pathways using techniques from biochemistry, molecular biology, and cell biology.
The model systems are vascular smooth muscle cell and ovarian cancer cell cultures as well as intact arteries, in situ tumors and human samples. The techniques used in these projects include cell culture, RNA isolation, quantitative RT-PCR, Western blotting, cell imaging, arterial dissection and tumor analysis. The results of these studies will give a better understanding of how vascular and tumor cells respond to their environment and whether transcriptional changes are subject to therapeutic intervention.
Ongoing Research Projects:
Regulation of angiogenesis by hypoxia in cancer cells and vascular smooth muscle cells
Calcium-regulated gene transcription in vascular smooth muscle cells during hypertension
Protein synthesis regulators as mediators of inflammation and angiogenesis in the tumor microenvironment
- Williams TF, Mirando AT, Wilkinson B, Francklyn CS, Lounsbury KM. Secreted Threonyl-tRNA Synthetase Stimulates Endothelial Cell Migration and Angiogenesis. Nature Sci Repts, 3:1317, 2013
- Wellman TL, Eckenstein M, Wong C, Rincon M, Ashitaga T, Mount SL, Francklyn CS, Lounsbury KM. Threonyl-tRNA synthetase overexpression correlates with angiogenic markers and progression of human ovarian cancer. BMC Cancer. 14: 620, 2014
- Mirando AC, Fang P, Williams TF, Baldor LC, Howe AK, Lounsbury KM, Francklyn CS. Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor. Nature Sci Repts. 5: 13160, 2015