Summary
Director, Microscopy Imaging Center: This is a College of Medicine, as well as Vermont Cancer Center Core Facility. The facility currently consists of a JEOL 1210 scanning transmission electron microscope with an attached X-ray microanalysis system, a JEOL 6060 scanning electron microscope, a Zeiss LSM 510 META confocal scanning laser microscope, a BioRad MRC 1024 confocal scanning laser microscope, an Olympus BX50 wide-field fluorescence imaging system with Optronics MagnaFire digital camera and Universal Imaging MetaMorph image analysis system, a Zeiss Axioscope wide-field fluorescence microscopy system, a CompuCyte laser scanning cytometer, an Arcturus laser capture microdissector, a Digital Instruments atomic force microscope, an Eppendorf microinjection system mounted on a n Olympus IX70 inverted one running Improvision Volocity software, and the other Universal Imaging MetaMorph. Also available in the faciltiy are a variety of flat-bed scanners and a Fujix Pictrography 3500 digital printer.
I am also responsible for directing the clinical electron microscopy in the Department of Pathology, and assuring compliance of the laboratory with College of Academic Pathologists (CAP) guidelines. The Microscopy Imaging Center was recently awarded accreditation by the Commission on Laboratory Accreditation of CAP.
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Research Overview
I am currently involved in studies using multimodal imaging techniques in investigations of cardiovascular disease and environmental pathology.
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Education
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University of Lowell (Massachusetts) B.S. Biological Sciences, 1980
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Kansas State University, M.S. Anatomy & Physiology 1982
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University of Basel (Switzerland), Ph.D. Cell Biology 1987
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Kansas State University, M.S. Anatomy & Physiology 1982
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Postdoctoral Training
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6/87-12/87 Post-Doctoral Fellow, Dept. of Cell Biology, Biocenter, University of Basel, Klingelbergstr. 70, 4056 Basel, Switzerland
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Positions Held
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5/02 Promoted to Professor, Department of Pathology, University of Vermont
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5/96 Promoted to Associate Professor, Department of Pathology, University of Vermont
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9/95 Secondary appointment as Assistant Professor, Vascular Biology Unit, Department of Medicine, College of Medicine, University of Vermont, Burlington, VT 05405
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7/94 Appointed as Director of Cell Imaging Facility, Department of Pathology, College of Medicine, University of Vermont, Burlington, VT 05405
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7/89-7/94 Assistant Professor and Director of Electron Microscopy Facility, Department of Pathology, University of Vermont, Burlington, VT 05405
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12/87-6/89 Research Associate and Director of Electron Microscopy Facility, Department of Pathology, University of Vermont, Burlington, VT 05405
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Publications
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Taatjes, D.J., Sobel, B.E., and Budd, R.C. (2008) Morphological and cytochemical determination of cell death by apoptosis. Histochem. Cell Biol. 129, 33-43.
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Sobel, B.E., Zaman, A.K.M.T., Budd, R.C., Schneider, D.J., and Taatjes, D.J. (2008) Attenuation of apoptosis and the eye of the beholder. Cor. Art. Dis. 19, 55-58.
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Manning, C.B., Sabo-Attwood, T., Robledo, R.F., MacPherson, M.B., Rincon, M., Vacek, P., Hemenway, D., Taatjes, D.J., Lee, P.J., and Mossman, B.T. (2008) Targeting the MEK1 cascade in lung epithelium inhibits proliferation and fibrogenesis by asbestos. Am. J. Respir. Cell Mol. Biol., In press.
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Hatle, K.M., Neveu, W., Dienz, O., Rymarchyk, S., Barrantes, R., Hale, S., Farley, N., Lounsbury, K.M., Bond, J.P., Taatjes, D. J., and Rincon, M. (2007) MCJ promotes c-Jun degradation to prevent ABCB1 transporter expression. Mol. Cell Biol 27, 2952-2966.
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Taatjes D.J., Wadsworth M.P., Zaman A.K.M.T., Schneider D.J., and Sobel B.E. (2007) A novel dual staining method for identification of apoptotic cells reveals a modest apoptotic response in infarcted mouse myocardium. Histochem. Cell Biol. 128, 275-283.
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Zuber, C., Taatjes, D.J., and Roth, J. (2007) Recent progress in histochemistry. Histochem. Cell Biol. 128, 557-594.
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Zaman A.K.M.T., Fujii S., Schneider D.J., Taatjes D.J., Lijnen H.R., and Sobel B.E. (2007) Deleterious effects of lack of cardiac PAI-1 after coronary occlusion in mice and their pathophysiologic determinants. Histochem. Cell Biol. 128, 135-145.
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Storch, N., Taatjes, D.J., Bouffard, N.A., Locknar, S., Bishop, N.M., and Langevin, H.M. (2007) Alpha-Smooth muscle actin distribution in cytoplasm and nuclear invaginations of connective tissue fibroblasts. Histochem. Cell Biol. 127, 523-530.
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Bostrom, M.A., Boyanovsky, B.B., Jordan, C.T., Wadsworth, M.P., Taatjes, D.J., de Beer, F.C., and Webb, N.R. (2007) GV sPLA2 promotes atherosclerosis: Evidence from genetically altered mice. Arterioscler. Thromb. Vasc. Biol. 27, 600-606.
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Shukla, A., Lounsbury, K.M., Barrett, T.F., Gell, J., Rincon, M., Butnor, K.J., Taatjes, D.J., Davis, G.S., Vacek, P., Nakayama, K.I., Nakayama, K., Steele, C., and Mossman, B.T. (2007) Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-delta knockout mice. Am. J. Pathol. 170, 140-151.
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Blumen, S.R., Cheng, K., Ramos-Nino, M., Taatjes, D.J., Weiss, D., Landry, C.C., and Mossman, B.T. (2007) Unique mechanisms of uptake of acid-prepared mesoporous spheres (APMS) by lung epithelial and mesothelioma cells. Am. J. Respir. Cell Mol. Biol. 36, 333-342.
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Trott, J.F., Farley, N.R., Taatjes, D.J., and Hovey, R.C. (2007) Cloning and functional characterization of allelic variation in the porcine prolactin receptor. Domest. Anim. Endocrinol. 33, 313-334.
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Jeremic, A., Quinn, A.S., Cho, W.J., Taatjes, D.J., and Jena, B.P. (2006) Energy-dependent disassembly of self-assembled SNARE complex: Observation at nanometer resolution using atomic force microscopy. J. Am. Chem. Soc. 128, 26-27.
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Langevin, H.M., Storch, K.N., White, S.L., Buttolph, T.R., and Taatjes, D.J. (2006) Fibroblast spreading induced by connective tissue stretch involves intracellular redistribution of alpha and beta actin. Histochem. Cell Biol. 125, 487-495.
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Pang, Y., von Turkovich, M., Wu, H., Mitchell, J., Mount, S., Taatjes, D.J., and Cooper, K. (2006) The binding of thyroid transcription factor 1 and hepatocyte paraffin 1 to mitochondrial proteins in hepatocytes: A molecular and immunoelectron microscopic investigation. Am. J. Clin. Path. 125, 722-726.
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Montigny, W.J., Quinn, A.S., Wu, X-X., Bovill, E.G., Rand, J.H., and Taatjes, D.J. (2006) Atomic force microscopy in the study of macromolecular interactions in hemostasis and thrombosis: Utility for investigation of the antiphophospholipid syndrome, In: Force Microscopy: Applications In Biology and Medicine (B.P. Jena and K.H. Horber, eds.), John Wiley & Sons, Inc., Hoboken, NJ, pp. 267-286.
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Wadsworth, M.P., Sobel, B.E., Schneider, D.J., Tra, W., van Hirtum, H., and Taatjes, D.J. (2006) Quantitative analysis of atherosclerotic lesion composition in mice, In: Cell Imaging Techniques (D.J. Taatjes and B.T. Mossman, eds.), Methods in Molecular Biology, Vol. 319, Humana Press, Totowa, NJ, pp. 137-152.
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Stern, M., Taatjes, D.J., and Mossman, B.T. (2006) Multifluorescence labeling techniques and confocal laser scanning microscopy on lung tissue, In: Cell Imaging Techniques (D.J. Taatjes and B.T. Mossman, eds.), Methods in Molecular Biology, Vol. 319, Humana Press, Totowa, NJ, pp. 67-76.
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Manning, C.B., Mossman, B.T., and Taatjes, D.J. (2006) Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse-transcriptase polymerase chain reaction (QRT-PCR), In: Cell Imaging Techniques (D.J. Taatjes and B.T. Mossman, eds.), Methods in Molecular Biology, Vol. 319, Humana Press, Totowa, NJ, pp. 231-236.
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Taatjes, D.J., Zuber, C., and Roth, J. (2006) The histochemistry and cell biology vade mecum: A review of 2005-2006. Histochem. Cell Biol. 126, 743-788.
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Poynter, M.E., Persinger, R., Irvin, C.G., Butnor, K.J., van Hirtum, H., Blay, W., Heintz, N.H., Robbins, J., Hemenway, D., Taatjes, D.J., and Janssen-Heininger, Y. (2006) Nitrogen dioxide enhances allergic airway inflammation and hyperresponsiveness in the mouse. Am. J. Physiol. Lung Cell. Mol. Physiol. 290, 144-152.
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Taatjes, D.J., and Roth, J. (2005) Recent progress in histochemistry and cell biology: The state of the art 2005. Histochem. Cell Biol. 124, 547-574.
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Haegens, A., van der Vliet, A., Butnor, K.J., Heintz, N., Taatjes, D., Hemenway, D.,Vacek, P., Freeman, B.A., Hazen, S.L., Brennan, M.L., and Mossman, B.T. (2005) Asbestos-induced lung inflammation and epithelial cell proliferation are altered in myeloperoxidase null mice. Cancer Res. 65, 9670-9677.
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Sabo-Attwood, T., Ramos-Nino, M., Bond, J., Butnor, K.J., Heintz, N., Gruber, A.D., Taatjes, D.J., Vacek, P., and Mossman, B.T. (2005) Gene expression profiles reveal increased mClca3 (Gob5) expression and mucin hypersecretion in a murine model of asbestos-induced fibrinogenesis. Am. J. Pathol. 167, 1243-1256.
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Clough MH, Schneider DJ, Sobel BE, White MF, Wadsworth MP, and Taatjes DJ: Attenuation of accumulation of neointimal lipid by pioglitazone in mice genetically deficient in insulin receptor substrate-2 and apolipoprotein E. J Histochem Cytochem 53:603-610, 2005.
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Mital, J., Schwarz, J., Taatjes, D.J., and Ward, G.E. (2005) Laser scanning cytometer-based assays for measuring host cell attachment and invasion by the human pathogen Toxoplasma gondii. Cytometry (Part A) 69A, 13-19.
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