Ratiometric Imaging: Description and Capabilities
The PTI Monochromator Ratiometric Imaging System
The PTI Monochromator system can image a wide variety of fluorescent probes. Currently, it is used for ratiometric imaging of Fura-2AM labeled preparations. The PTI DeltaRAM X Monochromator can generate excitation energy from 290-650 nm, with a continuously variable bandpass from 0-25 nm, depending on application. It can switch between wavelengths in less than 2 milliseconds to facilitate ratiometry.
Microscope
Nikon E600FN Upright Physiology Microscope
Lenses:
- Nikon Fluor 40X/0.80W DIC Physiology Dipping Lens WD 2
- Nikon Plan Flour 10x/0.30 Specimen Orientation Lens.
Monochromator
- PTI DeltaRAM with Xenon excitation source
Camera
- Photometrics Evolve EMCCD
Software