The Nikon TIRF – Total Internal Reflection Fluorescence Microscope
This system is designed to observe fluorescent signals in a thin region of samples adherent to a glass coverslip. An evanescent wave generated by TIR excites ~200nm above the glass/water interface, allowing observation of cell-surface phenomena.
Microscope
Nikon TE2000U:
- LUDL motorized filters
- Perfect focus module
- Conventional metal halide epifluorescence
Lenses
- Plan Apo TIRF 60X/1.45NA Oil lens with correction collar
- Nikon Plan Apo 20X/0.77
Laser
Coherent 50 mW 488 nm OPSL Sapphire laser
Camera
Photometrics Cascade 512B EMCCD
Computer
HP Compaq 8100 Elite 64-bit Workstation
Physiology Equipment
Axopatch 200B Integrating Patch Clamp