Electric Cell-substrate Impedance Sensing (ECIS)
An ECIS system (from Applied BioPhysics, Inc., Troy, NY) measures impedance, resistance, and capacitance in cultured cells grown on specially-fabricated chamber slides fitted with gold film-coated microelectrode. The cell electrical parameters are measured at various frequencies from 64-64,000 Hz, and indicate barrier function (adherence of cells to each other through junctional complexes), and adherence of cells to the substrate (capacitance). This system provides a continuous temporal assay of the physiologic state of cultured cells. The effect of the addition of chemical compounds, pharmaceuticals, or other reagents on cell physiology and morphology can then be monitored. The ECIS system in the MIC is housed adjacent to an Olympus IX70 inverted microscope, for convenient visual assessment of the cells.