Studies related to trauma induced coagulopathy

  1. Keyloun JW, Le TD, Orfeo T, Brummel-Ziedins KE, Bravo MC, Kaye MD, Bourne DE, Carney BC, Freeman K, Mann KG, Pusateri AE, Shupp JW; SYSCOT Study Group. Assessing Factor V Antigen and Degradation Products in Burn and Trauma Patients. J Surg Res. 2022;274:169-177. PMID: 35180493
  2. Keyloun JW, Lee TD, Pusateri AE, McLawhorn M, Bravo MC, Orfeo T, Johnson LS, Moffatt LT, Shupp JW. Inhalation Injury is Associated with Endotheliopathy and Abnormal Fibrinolytic Phenotypes in Burn Patients: A Cohort Study J Burn Care Res. 2021 Online ahead of print. PMID: 34089618
  3. Pusateri AE, Lee TD, Keyloun JW, Moffatt LT, Orfeo T, Brummel-Ziedins KE, McLawhorn M, Callcut R, Shupp JW; SYSCOT Study Group. Early Abnormal Fibrinolysis and Mortality in Patients with Thermal Injury: A Prospective Cohort Study BJS Open 2021 PMID:33893737
  4. Shupp JW, Brummel-Ziedins KE, Cohen MJ, Freeman K, Hammamieh R, Mudunuri US, Orfeo T, Moffatt LT, Brownstein BH, Mann KG, Jett M, Pusateri AE. Assessment of Coagulation Homeostasis in Blunt, Penetrating, and Thermal Trauma: Guidance for a Multicenter Systems Biology Approach. Shock. 2019 Oct;52(1S Suppl 1):84-91. PMID: 30339633.
  5. Bouchard BA, Orfeo T, Keith HN, Lavoie EM, Gissel M, Fung M, Mann KG. Microparticles formed during storage of red blood cell units support thrombin generation. J Trauma Acute Care Surg 2017 (Epub). PMID: 29251713.
  6. Gissel M, Brummel-Ziedins KE, Butenas S, Pusateri AE, Mann KG, Orfeo T. Effects of an acidic environment on coagulation dynamics. J Thromb Haemost 2016; 14:2001-2010. PMID: 27431334

Studies on coagulation dynamics

  1. Orfeo T, Brufatto N, Nesheim ME, Xu H, Butenas S, Mann KG, The factor V activation paradox. J Biol Chem 2004; 279:19580-19591. PMID: 14982929.
  2. Orfeo T, Butenas S, Brummel-Ziedins KE, Mann KG. The tissue factor requirement in blood coagulation. J Biol Chem 2005; 280:42887-42896. PMID: 16215234,  PMCID: PMC1369052.
  3. Orfeo T, Brummel-Ziedins KE, Gissel M, Butenas S, Mann KG. The nature of the stable blood clot procoagulant activities. J Biol Chem 2008; 283:9776-9786. PMID: 18268016, PMCID: PMC2442304
  4. Haynes LM, Dubief YC, Orfeo T, Mann KG. Dilutional control of prothrombin activation at physiologically relevant shear rates. Biophs J 2011: 100: 765-773. PMID: 21281592.
  5. Whelihan MF, Zachary V, Orfeo T, Mann KG. Prothrombin activation in blood coagulation: the erythrocyte contribution to thrombin generation. Blood 2012: 130:3837-3845. PMID: 22968460

Studies on various pharmacologic agents designed to suppress or aid blood clot formation and propagation

  1. Orfeo T, Butenas S, Brummel-Ziedins KE, Gissel M, Mann KG. Anticoagulation by factor Xa inhibitors. J Thromb Haemost 2010; 8:1745-1753. PMID: 20492473, PMCID: PMC2939944.
  2. Orfeo T, Gissel M, Butenas S, Undas A, Brummel-Ziedins KE, Mann KG. Anticoagulants and the propagation phase of thrombin generation. PLoS One 2011; 6:e27852. PMID: 22125631, PMCID: PMC3220702.
  3. Haynes LM, Orfeo T, Mann KG. Rivaroxaban delivery and reversal at a venous flow rate. Arterioscler Thromb Vasc Biol 2012; 32:2877-2883. PMID: 23023369, PMCID: PMC3629550.
  4. Orfeo T, Elsman R, Gissel M, Mann KG, Butenas S. Activation, activity and inactivation of factor VIII in factor VIII products.  Haemophilia. 2016; 22;462-473. PMID: 26822998
  5. Haynes LM, Orfeo T, Mann KG, Everse SJ, Brummel-Ziedins KE. Probing the dynamics of clot-bound thrombin at venous shear rates. Biophys J 2017; 112:1634-1644. PMID: 28445754.

Regulation of hemostatic processes by endothelial cells

  1. Shatos MA, Doherty JM, Orfeo T, Hoak J, Collen D, Stump D. Modulation of the fibrinolytic response of cultured human vascular endothelium by extracellularly generated oxygen radicals. J Biol Chem 1992; 267:597-601. PMID: 1730619.
  2. Orfeo T, Doherty JM, Adey G, Penar PL, Shatos MA. Sublethal percussion trauma in vitro causes a persisting derangement in the nonthrombogenic properties of brain endothelial cells. J Trauma 1994; 37: 347-357. PMID: 8083892.
  3. Shatos MA, Orfeo T, Doherty JM, Penar PL, Collen D, Mann KG. Alpha-thrombin stimulates urokinase production and DNA synthesis in cultured human cerebral microvascular endothelial cells. Arterioscler Thromb Vasc Biol 1995; 15:903-911. PMID: 7600122.
  4. Orfeo T, Gissel M, Haynes LM, Pusateri A, Mann KG, Brummel-Ziedins KE. Hemodilution and endothelial cell regulation of whole blood coagulation. Mil Med. 2018 183(suppl_1):175-182. PMID: 29635586


  1. Orfeo T, Gissel M, Butenas S, Undas A, Brummel-Ziedins KE, Mann KG. Anticoagulants and the propagation phase of thrombin generation. PLoS One 2011; 6:e27852. PMID: 22125631, PMCID: PMC3220702.
  2. Danforth CM, Orfeo T, Everse SJ, Mann KG, Brummel-Ziedins KE. Defining abnormal within the normal:  investigations into blood coagulation. PLoS One 2012; 7:e30385. PMID: 22319567, PMCID: PMC3271084.
  3. Wu TB, Wu S, Buoni M, Orfeo T, Brummel-Ziedins K, Cohen M, Petzold L. Computational model for hyperfibrinolytic onset of acute traumatic coagulopathy. Ann Biomed Eng. 2018 46:1173-1182. PMID: 29675813
  4. Rachel LeCover, Thomas Orfeo, Kathleen Brummel-Ziedins, Maria Bravo, Anthony Pusateri, and Jeffrey Varner. Kinetic modeling of coagulation and fibrinolysis. IFAC-PapersOnLine, 52(26):94{100, 2019. 
  5. Wu TB, Orfeo T, Moore HB, Sumislawski JJ, Cohen MJ, Petzold LR. Computational model of tranexamic acid on urokinase mediated fibrinolysis. PLoS One. 2020 May 26;15(5): e0233640. doi: 10.1371/journal.pone.0233640. PMID: 32453766
Complete List of Published Work in MyBibliography:

Dr. Thomas Orfeo